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Anchoring Junctions

Anchoring Junctions

Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...

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Related Experiment Video

Updated: May 13, 2026

Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures

Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures

Published on: July 27, 2022

Inverting adherent cells for visualizing ECM interactions at the basal cell side.

Tetyana Gudzenko¹, Clemens M Franz

¹DFG-Center for Functional Nanostructures, Karlsruhe Institute of Technology-KIT, Wolfgang-Gaede-Strasse 1a, 76131 Karlsruhe, Germany.

Ultramicroscopy

|March 5, 2013

Summary

This summary is machine-generated.

Researchers developed a cell inversion technique to study how cells interact with the extracellular matrix (ECM). This method allows high-resolution imaging of the basal cell side, revealing how nanoscale matrix interactions influence cell behavior and morphology.

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Last Updated: May 13, 2026

Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures

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Published on: July 27, 2022

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In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells

Published on: July 23, 2010

Area of Science:

Cell biology
Biophysics
Materials science

Background:

Cellular functions like migration and invasion are governed by extracellular matrix (ECM) interactions.
Basal cell-ECM interactions are crucial but difficult to study with high-resolution imaging techniques like AFM and SEM due to their location.

Purpose of the Study:

To develop a novel method for inverting adherent cells to expose the basal cell membrane for direct analysis.
To enable high-resolution imaging of nanoscale cell-matrix interactions at the basal cell surface.

Main Methods:

A fast and reliable cell inversion technique was established.
Inverted cells were analyzed using atomic force microscopy (AFM), scanning electron microscopy (SEM), and fluorescence microscopy.
The preservation of molecular features of ECM proteins and adhesion sites was confirmed.

Main Results:

The cell inversion method successfully exposed the basal cell membrane and associated ECM proteins for analysis.
Fibroblasts were observed to remodel fibronectin matrices from above but actively invade collagen matrices from both basal and apical sides.
Nanoscale ECM interactions, such as contact with collagen nanofibrils, were correlated with enhanced cell spreading and flattening.

Conclusions:

The cell inversion technique provides unprecedented insight into nanoscale cell-matrix interactions at the basal cell side.
This method facilitates the study of how ECM topography and composition influence cellular behavior and morphology.
The findings highlight the importance of nanoscale ECM features in regulating macroscopic cell responses.