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Updated: May 13, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy (Conpokal) on Live Cells

Published on: August 11, 2020

Single-image far-field subdiffraction limit imaging with axicon.

Craig Snoeyink¹, Steve Wereley

¹Department of Mechanical Engineering, Texas Tech University, Lubbock, Texas 79409-1021, USA. craig.snoeyink@ttu.edu

|March 5, 2013

Summary

Bessel beam microscopy (BBM) enhances microscope resolution by one-third using simple optics. This technique achieves subdiffraction limit imaging, resolving features as small as 110 nm in a single, broadband image.

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Area of Science:

Optics and Photonics
Microscopy
Biomedical Imaging

Background:

Traditional microscopy is limited by diffraction, restricting resolution.
Super-resolution techniques often require complex setups or specific labeling.

Purpose of the Study:

To introduce Bessel beam microscopy (BBM) as a novel super-resolution imaging technique.
To demonstrate BBM's ability to improve resolution beyond the diffraction limit.

Main Methods:

Integration of an axicon and lens into a standard microscope's optical path.
Utilizing broadband, unstructured illumination for imaging.
Experimental validation with subdiffraction limit fluorescent beads and angular diffraction simulations.

Main Results:

Achieved a one-third improvement in resolution over the base microscope.
Experimentally resolved closely packed subdiffraction limit fluorescent beads.
Simulations indicated the potential to resolve features as small as 110 nm.

Conclusions:

BBM offers a simple, cost-effective method for subdiffraction limit imaging.
The technique captures high-resolution information in a single image without specialized illumination.
BBM presents a unique approach to super-resolution microscopy using static geometric optics.