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Updated: May 13, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
Published on: April 7, 2014
Optical model enabling the use of Abbe-type refractometers on turbid suspensions
Augusto García-Valenzuela1, Humberto Contreras-Tello
1Centro de Ciencias Aplicadas y Desarrollo Tecnológico, Universidad Nacional Autónoma de México, Distrito Federal 04510, Mexico. augusto.garcia@ccadet.unam.mx
Abstract:
We derive a simple model for the angular-intensity profiles of diffuse light transmitted from a turbid colloid into a transparent medium of higher refractive index (RI) near the critical angle. Adjusting this model to experimental profiles obtained with an Abbe-type refractometer offers a sensitive and robust way of measuring the complex effective RI of highly scattering media.

