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Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
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Published on: June 19, 2018

Perspective: pre-chemistry conformational changes in DNA polymerase mechanisms

Tamar Schlick1, Karunesh Arora, William A Beard

  • 1Department of Chemistry, New York University, 100 Washington Square East, Silver Building, New York, NY 10003, USA. Courant Institute of Mathematical Sciences, New York, University, 251 Mercer Street, New York, NY 10012, USA.

Theoretical Chemistry Accounts
|March 6, 2013
PubMed
Summary

Substrate-induced conformational changes are crucial for DNA polymerase beta (Pol β) to assemble its active site before catalysis. These pre-chemistry adjustments ensure accurate DNA synthesis and high fidelity.

Keywords:
Catalytic cycle chemical stepDNA polymerase βEnzyme catalysisIntrinsic protein dynamicsNucleotidyl transferPre-chemistry conformational adjustments

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