Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hepatitis01:25

Hepatitis

Hepatitis is an inflammatory condition of the liver most commonly caused by hepatotropic viruses (A–E), though non-infectious causes such as alcohol and drugs also exist.Hepatitis AHepatitis A virus (HAV) is a non-enveloped RNA virus of the Picornaviridae family. It is primarily transmitted via the fecal-oral route, typically through ingestion of contaminated food or water. After ingestion, HAV enters the bloodstream through the oropharynx or intestinal epithelium and reaches the liver. The...
Viral Hepatitis I: Introduction01:28

Viral Hepatitis I: Introduction

Viral hepatitis is an inflammatory condition of the liver caused by infection with hepatotropic viruses, most commonly hepatitis A, B, C, D, and E. Despite variations in structure and transmission, all viruses mentioned infect hepatocytes and provoke immune responses that can hinder liver function. Additionally, some non-hepatotropic viruses can also lead to hepatic inflammation.Hepatitis A VirusHepatitis A virus (HAV) is transmitted through the fecal–oral route, typically by ingestion of food...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intra-host viral population dynamics during acute hepatitis E virus infection.

mBio·2026
Same author

Hantavirus stability and inactivation.

PLoS neglected tropical diseases·2026
Same author

Cystobactamid off-target profiling reveals favorable safety, superoxide reduction, and SCARB1 inhibition in eukaryotes.

npj drug discovery·2026
Same author

Respiratory syncytial viral load drives ciliated cell dedifferentiation and suppresses antiviral immunity.

Science advances·2026
Same author

Augmenting hepatitis C E1E2 glycoprotein antibody detection via an alternative lectin-assisted mannose-binding ELISA.

Virology·2026
Same author

Exploration of the relationship between laboratory stress parameters and leukocyte coping capacity in ponies exposed to repetitive handling during a research project.

BMC veterinary research·2026

Related Experiment Video

Updated: May 13, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
13:04

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

Cell culture systems for hepatitis C virus.

Eike Steinmann1, Thomas Pietschmann

  • 1Helmholtz Centre for Infection Research, Hannover, Germany.

Current Topics in Microbiology and Immunology
|March 7, 2013
PubMed
Summary

Developing effective cell culture models for hepatitis C virus (HCV) is crucial for understanding viral replication and host interactions. Advances in HCV cell culture have significantly aided research and drug development.

Area of Science:

  • Virology
  • Cell Biology
  • Hepatitis C Research

Background:

  • Viruses require cell culture systems for propagation and studying virus-host interactions.
  • Hepatitis C virus (HCV) cell culture development has been historically challenging.
  • Early HCV culture efforts had limited success prior to the virus's molecular cloning in 1989.

Purpose of the Study:

  • To review the breakthroughs in developing permissive and authentic cell culture models for HCV.
  • To highlight the impact of these models on understanding HCV replication and host interplay.
  • To discuss future directions for refining cell culture models for comprehensive HCV research.

Main Methods:

  • Review of historical and recent advancements in HCV cell culture techniques.

More Related Videos

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
10:25

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

Published on: February 19, 2019

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
10:28

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

Published on: June 26, 2020

Related Experiment Videos

Last Updated: May 13, 2026

A Protocol for Analyzing Hepatitis C Virus Replication
13:04

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection
10:25

"Liver-on-a-Chip" Cultures of Primary Hepatocytes and Kupffer Cells for Hepatitis B Virus Infection

Published on: February 19, 2019

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
10:28

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

Published on: June 26, 2020

  • Analysis of how cell culture models have enabled study of viral RNA replication, entry, and complete replication cycle.
  • Discussion of complementary cell-based systems for specific steps in HCV replication.

    • Main Results:

    • Significant breakthroughs have enabled the analysis of the complete HCV replication cycle in cultured cells since 2005.
    • Developed cell culture models have greatly benefited basic and applied HCV research.
    • These models have facilitated drug development and deepened understanding of HCV replication determinants.

    Conclusions:

    • HCV cell culture models have been instrumental in advancing our knowledge of viral biology and host interactions.
    • Refining models to mimic natural hepatocytes will likely reveal novel aspects of HCV replication.
    • Future models for culturing diverse HCV genotypes could elucidate viral adaptations and disease variability.