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Laccase production by Coriolopsis caperata RCK2011: optimization under solid state fermentation by Taguchi DOE
Preeti Nandal1, Sreenivas Rao Ravella, Ramesh Chander Kuhad
1Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, New Delhi-110021, India.
Scientific Reports
|March 7, 2013
Summary
Solid-state fermentation optimized laccase production using Coriolopsis caperata RCK2011. Taguchi methods identified key factors, enhancing enzyme yield by 58.01% for commercial potential.
Area of Science:
- Biotechnology
- Enzyme Engineering
- Industrial Microbiology
Background:
- Laccase is a versatile enzyme with numerous industrial applications.
- Optimizing laccase production is crucial for cost-effective industrial use.
- Solid-state fermentation (SSF) offers advantages for fungal enzyme production.
Purpose of the Study:
- To optimize laccase production by Coriolopsis caperata RCK2011 using solid-state fermentation.
- To identify the most influential factors affecting laccase yield.
- To assess the commercial potential of the optimized process.
Main Methods:
- Taguchi design of experiment (DOE) with an L18 orthogonal array was employed.
- Eight influential factors were investigated using Qualitek-4 software.
- Optimized conditions were validated through experimental runs.
Main Results:
- pH was the most significant factor at the individual level.
- Corn steep liquor (CSL), biotin, and KH2PO4 showed significant interactive effects.
- Optimal conditions yielded 1623.55 Ugds(-1) laccase, a 58.01% improvement.
Conclusions:
- The study successfully optimized laccase production by Coriolopsis caperata RCK2011.
- The identified optimal conditions demonstrate significant potential for industrial-scale enzyme production.
- Coriolopsis caperata RCK2011 shows commercial viability for laccase production.
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