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Related Concept Videos

Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
Simple Trusses01:21

Simple Trusses

A truss is a structural framework consisting of slender members connected at joints, designed to support external loads while minimizing material usage and weight. Simple trusses are a type of planar truss where all members lie within a single two-dimensional plane.
The most basic planar truss is a simple truss with three members arranged in a triangular formation. This triangular truss is inherently stable and rigid due to its geometry, making it an ideal starting point for creating more...
Space Trusses01:25

Space Trusses

A space truss is a three-dimensional counterpart of a planar truss. These structures consist of members connected at their ends, often utilizing ball-and-socket joints to create a stable and versatile framework. The space truss is widely used in various construction projects due to its adaptability and capacity to withstand complex loads.
At the core of a space truss lies the fundamental unit known as the tetrahedron. This structure is composed of six members that form a three-dimensional shape...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...

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Related Experiment Video

Updated: May 13, 2026

Translating Ribosome Affinity Purification (TRAP) to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale
09:41

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Published on: May 14, 2020

Trs20 is required for TRAPP II assembly.

David Taussig1, Zhanna Lipatova, Jane J Kim

  • 1Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL, USA.

Traffic (Copenhagen, Denmark)
|March 8, 2013
PubMed
Summary
This summary is machine-generated.

Trs20 is essential for assembling the TRAPP II complex, which activates Golgi Ypt/Rab GTPases. A SEDT-linked mutation impairs Trs20 function, disrupting TRAPP II assembly and highlighting a conserved role in Golgi trafficking.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • TRAPP complexes are key regulators of Golgi Ypt/Rab GTPase activity.
  • TRAPP I is well-characterized, but TRAPP II function and assembly remain poorly understood.
  • Trs20 is an essential subunit for both TRAPP I and TRAPP II, yet its specific role in TRAPP II is unclear.

Purpose of the Study:

  • To elucidate the role of Trs20 in TRAPP II assembly and function.
  • To investigate the impact of SEDT-linked mutations on Trs20 activity.
  • To understand the conserved function of Trs20 in Golgi trafficking.

Main Methods:

  • Co-immunoprecipitation assays to study protein interactions.
  • In vitro nucleotide exchange assays to assess GTPase activity.
  • Analysis of TRAPP complex assembly in yeast and human cell models.

Main Results:

  • Trs20 directly interacts with the TRAPP II-specific subunit Trs120.
  • This Trs20-Trs120 interaction is critical for TRAPP II assembly and Ypt32 nucleotide exchange activity.
  • A SEDT-associated mutation (Trs20-D46Y) disrupts TRAPP II assembly by preventing Trs120 interaction.

Conclusions:

  • Trs20 plays a crucial, conserved role in the assembly of the TRAPP II complex.
  • Defective TRAPP II assembly due to SEDT mutations underlies the cartilage disorder.
  • Understanding Trs20 function provides insights into Golgi organization and related diseases.