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Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation
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The low dose gamma ionising radiation impact upon cooperativity of androgen-specific proteins

Gennady N Filchenkov1, Eugene H Popoff, Alexander D Naumov

  • 1Lab. of Biochemistry, Institute of Radiobiology, National Academy of Sciences of Belarus, Feduninskogo 4, Gomel 246007, Belarus.

Journal of Environmental Radioactivity
|March 8, 2013
PubMed

Keywords:
(h)TeBG5α-dihydrotestosteroneAABPACARARCAndrogenic receptorAndrogensBB(max)B(nsp)B(t)ChNPPChernobyl nuclear power plantDCCDFDHTHbHexokinaseIRISSInternational Space StationIonising radiationK(a)K(d)MFMTOFPMSISTT(add)Testosterone-binding globulinUaaadenylate cyclaseamino acidsandrogen receptorandrogen receptor complexandrogen(s)androgen-binding protein of rat i.e. (r)TeBGdextran-coated charcoal (1:9)dimerization factorequilibrium association constantequilibrium dissociation constantexternal plasma(tic) membrane of cellfinal concentration of [(3)H]-DHT added in incubation systemhemoglobinhuman testosterone-binding globulinionising radiationmaximal B′ value calculated by approximation in Scatchard plotmethyltrienolonemonomerization factornonspecific binding of Aoligomerization factorquantity of specifically bound A ([(3)H]-DHT by TeBG and [(3)H]-MT by RA)standard incubation systemtestosteronethe Hill coefficient indicating a degree of cooperativity for A-binding by TeBG or AR (calculated in Hill plot, see Figs. 2 and 3)total binding of [(3)H]-Aunbound [(3)H]-A that removed by DCC after incubationη(Hill)

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