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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Updated: May 13, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

Pre-analytical variables in miRNA analysis.

Nils Becker¹, Christina M Lockwood

¹Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, MO, USA.

Clinical Biochemistry

|March 8, 2013

Summary

This summary is machine-generated.

MicroRNAs (miRNAs) are crucial biomarkers for diseases like cancer. This review details pre-analytical variables impacting miRNA detection in blood and tissues, ensuring accurate diagnostic results.

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Last Updated: May 13, 2026

mirMachine: A One-Stop Shop for Plant miRNA Annotation

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Published on: May 1, 2021

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

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Published on: April 14, 2015

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Area of Science:

Molecular Biology
Biochemistry
Genetics

Background:

MicroRNAs (miRNAs) are short non-coding RNAs regulating cellular processes.
Differential expression of miRNAs is observed in neoplasia and other diseases.
miRNAs show potential as diagnostic and prognostic biomarkers in various body fluids, particularly blood.

Purpose of the Study:

To comprehensively review pre-analytical variables affecting miRNA analysis.
To highlight variables specific to circulating and tissue miRNA detection.
To ensure reliable miRNA profiling for clinical applications.

Main Methods:

Systematic literature review of pre-analytical variables in miRNA analysis.
Focus on factors influencing detection in general miRNA analysis.
Specific examination of variables impacting circulating and tissue miRNA detection.

Main Results:

Numerous pre-analytical variables can significantly influence miRNA detection accuracy.
Erroneous results due to pre-analytical factors can alter patient diagnosis and treatment.
Systematic studies on pre-analytical variables are scarce but critical.

Conclusions:

Standardizing pre-analytical procedures is essential for reliable miRNA biomarker utility.
Understanding and controlling pre-analytical variables is crucial for clinical translation of miRNA diagnostics.
Further systematic research is needed to optimize miRNA detection methods.