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Updated: May 13, 2026

A Method of Targeted Cell Isolation via Glass Surface Functionalization

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

Selective individual primary cell capture using locally bio-functionalized micropores.

Jie Liu¹, Radoslaw Bombera, Loïc Leroy

¹Institut Nanosciences et Cryogénie, UMR5819 CEA/CNRS/UJF, Grenoble, France.

|March 8, 2013

Summary

Bio-functionalized solid-state micropores selectively capture specific B or T lymphocytes. This method enables cell identification and isolation, even in complex mixtures with overlapping cell dimensions.

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Cell Capture Using a Microfluidic Device

Cell Capture Using a Microfluidic Device

Published on: October 1, 2007

Area of Science:

Biotechnology
Cell Biology
Nanotechnology

Background:

Solid-state micropores have been used for cell recognition for decades.
The resistive-pulse technique struggles with differentiating cells of similar sizes, like B and T lymphocytes.
Specific cell capture in micropores offers an alternative for cell differentiation.

Purpose of the Study:

To develop a method for specific cell capture using bio-functionalized micropores.
To overcome limitations of existing cell sizing techniques for closely related cell types.
To enable identification of unlabeled cells through selective trapping.

Main Methods:

Micropore inner walls were functionalized with antibodies targeting B or T lymphocyte surface proteins.
Contactless electro-functionalization formed polypyrrole-oligodeoxynucleotide (ODN) copolymer films.
Selective trapping was validated using microbeads and mouse splenocytes.

Main Results:

Bio-functionalized micropores selectively immobilized B or T lymphocytes.
Successful trapping of target cells was confirmed via optical microscopy and resistive-pulse analysis.
The technique demonstrated the ability to isolate cells based on specific surface markers.

Conclusions:

Locally bio-functionalized micropores can effectively isolate target cells from suspension.
This method allows for cell isolation during translocation, even within complex mixtures.
The approach enhances cell recognition capabilities for cells with similar dimensions.