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Related Experiment Video

Updated: May 13, 2026

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

An Orthotopic Bladder Cancer Model for Gene Delivery Studies

Published on: December 1, 2013

Bladder polypoid cystitis-derived A20 associates with tumorigenesis.

Ming Wang¹, Shijian Li

¹Department of Urology, The First Affiliated Hospital, Chongqing Medical University, Chongqing, 400016, China, mingwang343@163.com.

Cell Biochemistry and Biophysics

|March 9, 2013

Summary

The ubiquitin E3 ligase A20 (A20) correlates with bladder cancer development by suppressing the anticancer gene p53. High A20 levels in bladder tissues indicate increased tumor risk and progression.

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Induction of Invasive Transitional Cell Bladder Carcinoma in Immune Intact Human MUC1 Transgenic Mice: A Model for Immunotherapy Development

Published on: October 30, 2013

Area of Science:

Oncology
Molecular Biology
Urology

Background:

Chronic inflammation in the bladder is linked to bladder cancer pathogenesis, but mechanisms remain unclear.
The PT53 gene, an important anticancer gene, is often suppressed in cancerous cells.
Ubiquitin E3 ligase A20 (A20) influences epithelial cell regulation.

Purpose of the Study:

To investigate the correlation between A20 and the pathogenesis of bladder cancer.
To explore the role of A20 in the suppression of the p53 gene.

Main Methods:

Analysis of A20 and p53 levels in human bladder cancer, polypoid cystitis, and chronic inflammation tissues using quantitative real-time RT-PCR and Western blotting.
Immune precipitation assays to detect A20-p53 complex formation.
In vitro experiments using HEK293 cells to assess the effect of A20 overexpression on p53 levels.

Main Results:

Elevated A20 levels were observed in human bladder cancer and polypoid tissues, correlating positively with bladder tumorigenesis.
A significant proportion (26.1%) of patients with bladder polypoid cystitis were diagnosed with bladder cancer.
A20 formed complexes with p53 in bladder cancer and polypoid tissues, and A20 overexpression suppressed p53 protein levels in HEK293 cells.

Conclusions:

A20 is positively correlated with the tumorigenesis of bladder polypoid disorders.
A20 may contribute to bladder cancer development by binding to and suppressing the tumor suppressor p53.