Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
Whole Body Regeneration01:33

Whole Body Regeneration

Regeneration is the process of restoring injured or lost tissues, organs, or body parts. While simpler organisms generally show greater ability to regenerate their whole body, few complex animals show similarly exceptional regeneration. For example, planarian flatworms have a unique regenerative potential making them a popular study organism among biologists to understand the mechanisms of whole body regeneration. Other organisms, such as hydra, also show extreme regeneration potential; even...
Overview of Regeneration and Repair01:19

Overview of Regeneration and Repair

Regeneration and repair processes are critical in healing damages caused by injury, disease, and aging. In regeneration, the damaged tissue is entirely replaced with new growth that restores the original architecture and function. In contrast, tissue repair usually results in a fixed tissue architecture involving scar formation. Scars generally do not reestablish tissue function and may also exhibit structural abnormalities at the injury site.
Regeneration
All animals have varying degrees of...
iPS Cell Differentiation01:22

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Understanding Outcomes in Hemodialysis Patients Who Underwent Transcatheter Aortic Valve Replacement With the Latest Devices.

JACC. Asia·2026
Same author

Health Status Changes and Long-Term Clinical Outcomes in Patients With Atrial Fibrillation.

JAMA network open·2026
Same author

Sex differences in circulating microRNA profiles in heart failure with preserved and reduced ejection fraction.

European heart journal open·2026
Same author

Serial Assessment of Patient-Reported Health Status and Subsequent Clinical Outcomes in Atrial Fibrillation.

Circulation journal : official journal of the Japanese Circulation Society·2026
Same author

7-Year Outcomes of Balloon-Expandable Versus Self-Expanding Valves in Women Undergoing Transcatheter Aortic Valve Replacement.

JACC. Asia·2026
Same author

Percutaneous closure strategy in patients with patent foramen ovale and coexisting small atrial septal defect: a single-center experience.

Cardiovascular intervention and therapeutics·2026

Related Experiment Video

Updated: May 13, 2026

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes
09:16

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

Heart regeneration using reprogramming technology.

Masaki Ieda1

  • 1Department of Clinical and Molecular Cardiovascular Research, Keio University School of Medicine, Tokyo, Japan. mieda@z8.keio.jp

Proceedings of the Japan Academy. Series B, Physical and Biological Sciences
|March 12, 2013
PubMed
Summary
This summary is machine-generated.

Scientists directly reprogram fibroblasts into functional cardiomyocytes using three cardiac transcription factors. This direct reprogramming offers a promising new avenue for heart regeneration therapies, bypassing the need for induced pluripotent stem cells (iPSCs).

More Related Videos

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
10:52

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

Published on: January 19, 2020

Related Experiment Videos

Last Updated: May 13, 2026

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes
09:16

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
10:52

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

Published on: January 19, 2020

Area of Science:

  • Regenerative Medicine
  • Cardiovascular Research
  • Stem Cell Biology

Background:

  • Heart disease leads to irreversible loss of cardiomyocytes, with limited therapeutic options.
  • Stem cell-derived cardiomyocytes offer potential for cardiac repair, building on induced pluripotent stem cells (iPSCs) advancements.
  • Direct cellular conversion, bypassing pluripotency, is an emerging strategy in regenerative medicine.

Purpose of the Study:

  • To investigate the direct reprogramming of fibroblasts into functional cardiomyocytes.
  • To explore the efficacy of specific cardiac transcription factors in direct cell conversion.
  • To review the potential of reprogramming technology for heart regeneration.

Main Methods:

  • Overexpression of a combination of three cardiac transcription factors: Gata4, Mef2c, and Tbx5.
  • In vitro and in vivo experimental models to assess cardiomyocyte induction.
  • Review of advancements in cell therapy, differentiation protocols, and transplantation.

Main Results:

  • Functional cardiomyocytes were successfully induced directly from fibroblasts.
  • The combination of Gata4, Mef2c, and Tbx5 facilitated direct cardiac reprogramming.
  • Demonstrated feasibility of direct reprogramming both in vitro and in vivo.

Conclusions:

  • Direct reprogramming of fibroblasts into cardiomyocytes is achievable using specific transcription factors.
  • This approach bypasses the induced pluripotent stem cell (iPSC) stage, offering a potentially more efficient regenerative strategy.
  • Reprogramming technology holds significant promise for future heart regeneration therapies.