Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proofreading01:43

Proofreading

52.0K
Overview
52.0K
ATP and Macromolecule Synthesis01:28

ATP and Macromolecule Synthesis

5.1K
Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
5.1K
Proofreading01:31

Proofreading

7.5K
Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase...
7.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure of the pre-initiation complex explains CMGE biogenesis.

Nature·2026
Same author

Targeting cancer-specific mutations with RNA-triggered chromatin shredding.

Nature·2026
Same author

Selective Elimination of TP53 Mutant Cells by Transcript-Activated Chromatin Shredding.

bioRxiv : the preprint server for biology·2026
Same author

DNA translocation by the CMG helicase: the helical inchworm model.

Biochemical Society transactions·2026
Same author

RNA polymerase II is a polar roadblock to a progressing DNA fork.

Nature communications·2025
Same author

Biochemical reconstitution of sister chromatid cohesion establishment during DNA replication.

Molecular cell·2025

Related Experiment Video

Updated: Apr 28, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

14.6K

ATPase-dependent quality control of DNA replication origin licensing.

Jordi Frigola1, Dirk Remus, Amina Mehanna

  • 1Cancer Research UK London Research Institute, Clare Hall Laboratories, South Mimms EN6 3LD, UK.

Nature
|March 12, 2013
PubMed
Summary

Precise DNA replication relies on loading the Mcm2-7 DNA helicase. A conserved Mcm3 domain recruits Mcm2-7 to origins by stimulating ORC-Cdc6 ATPase activity, ensuring replication occurs only once per cell cycle.

More Related Videos

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

8.2K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

3.9K

Related Experiment Videos

Last Updated: Apr 28, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

14.6K
Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis
11:08

Proofreading and DNA Repair Assay Using Single Nucleotide Extension and MALDI-TOF Mass Spectrometry Analysis

Published on: June 19, 2018

8.2K
DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

3.9K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Eukaryotic DNA replication requires precise loading of the Mcm2-7 DNA helicase at replication origins.
  • Origin licensing, mediated by the origin recognition complex (ORC), Cdc6, and Cdt1, is crucial for once-per-cell-cycle replication but its mechanism remains unclear.

Purpose of the Study:

  • To elucidate the molecular mechanism of Mcm2-7 helicase loading during origin licensing in Saccharomyces cerevisiae.
  • To identify the role of Mcm3 in the recruitment of Mcm2-7 to replication origins.

Main Methods:

  • Investigated the interaction between Mcm3 and the ORC-Cdc6 complex.
  • Assessed the effect of Mcm3 on ORC-Cdc6 ATPase activity.
  • Analyzed the impact of ATP hydrolysis on Mcm2-7 loading and release.

Main Results:

  • Identified an essential, conserved carboxy-terminal domain of Mcm3 that interacts with ORC-Cdc6.
  • Demonstrated that this Mcm3 domain stimulates the ATPase activity of ORC-Cdc6.
  • Showed that ATP hydrolysis by ORC-Cdc6 is critical for Mcm2-7 loading but can also lead to release under specific conditions.

Conclusions:

  • The Mcm3 carboxy-terminal domain is a key factor in recruiting Mcm2-7 hexamers to replication origins.
  • A novel ATPase-dependent mechanism involving ORC-Cdc6 stimulation by Mcm3 regulates Mcm2-7 loading and release.
  • This mechanism contributes to the precise, once-per-cell-cycle DNA replication in eukaryotes.