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Updated: May 13, 2026

Autonomously Bioluminescent Mammalian Cells for Continuous and Real-time Monitoring of Cytotoxicity
Published on: October 28, 2013
Bioluminescent assays for cytochrome P450 enzymes
Douglas S Auld1, Henrike Veith, James J Cali
1Novartis Institutes for Biomedical Research, Cambridge, MA, USA.
Abstract:
The cytochrome P450 (CYP) family contains 57 enzymes in humans. The activity of CYPs against xenobiotics is a primary consideration in drug optimization efforts. Here we describe a series of bioluminescent assays that enable the rapid profiling of CYP activity against compound collections. The assays employ a coupled-enzyme format where firefly luciferase is used to measure CYP enzyme activity through metabolism of pro-luciferase substrates.
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