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Related Experiment Video

Updated: May 13, 2026

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems
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Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

Rapid LC-MS drug metabolite profiling using bioreactor particles.

Linlin Zhao1, Besnik Bajrami, James F Rusling

  • 1Department of Chemistry, University of Connecticut, Storrs, CT, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 12, 2013
PubMed
Summary
This summary is machine-generated.

Enzyme-coated magnetic particles offer a rapid method for metabolic screening and metabolite profiling. This approach allows for high-throughput analysis of numerous reactions and DNA damage studies.

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Last Updated: May 13, 2026

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Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS)

Published on: March 14, 2013

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Molecular Biology

Background:

  • Metabolic screening and metabolite profiling are crucial for understanding biological processes.
  • Current methods can be time-consuming and require complex sample handling.
  • There is a need for faster, more efficient analytical techniques.

Purpose of the Study:

  • To develop a semi-automated, high-throughput method for metabolite profiling using enzyme-coated magnetic bioreactor particles.
  • To enable rapid investigation of enzymatic reactions and determination of DNA damage and adducts.

Main Methods:

  • Utilized enzyme-coated magnetic bioreactor particles in a 96-well plate format.
  • Employed liquid chromatography-tandem mass spectrometry (LC-MS/MS) for detection.
  • Developed a semi-automated workflow for reaction analysis.
  • Incorporated DNA into particle films for damage assessment.

Main Results:

  • Demonstrated a fast, convenient approach to metabolic screening.
  • Enabled investigation of reactions within 1- to 2-min periods.
  • Achieved parallel processing of 96 or more reactions or time points.
  • Facilitated determination of DNA damage rates and metabolite-DNA adduct structures.

Conclusions:

  • Enzyme-coated magnetic bioreactor particles provide an efficient platform for advanced metabolite profiling.
  • The developed semi-automated LC-MS/MS technique significantly enhances throughput for biochemical assays.
  • This method is applicable to both metabolic studies and the analysis of genotoxic events.