Related Experiment Video
Updated: May 13, 2026

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes
Published on: March 3, 2023
TEM-187, a new extended-spectrum β-lactamase with weak activity in a Proteus mirabilis clinical strain
Stéphane Corvec1, Racha Beyrouthy, Lise Crémet
1Service de Bactériologie-Hygiène, CHU de Nantes, Institut de Biologie, Nantes, France. stephane.corvec@chu-nantes.fr
Abstract:
A Proteus mirabilis clinical strain (7001324) was isolated from urine sample of a patient hospitalized in a long-term-care facility. PCR and cloning experiments performed with this strain identified a novel TEM-type β-lactamase (TEM-187) differing by four amino acid substitutions (Leu21Phe, Arg164His, Ala184Val, and Thr265Met) from TEM-1. This characterization provides further evidence for the diversity of extended-spectrum β-lactamases (ESBL) produced by P. mirabilis and for their potential spread to other Enterobacteriaceae due to a lack of sensitive detection methods used in daily practice.
Insights
A novel TEM-type beta-lactamase, TEM-187, was discovered in a Proteus mirabilis strain from a long-term-care facility. This finding highlights the growing diversity of beta-lactamases and their potential spread.
Area of Science:
- Microbiology
- Molecular Biology
- Antimicrobial Resistance
Background:
- Proteus mirabilis is a common cause of urinary tract infections, particularly in healthcare settings.
- The emergence of antibiotic resistance, especially beta-lactamases, poses a significant threat to public health.
- Extended-spectrum beta-lactamases (ESBLs) confer resistance to a wide range of antibiotics.
Related Concept Videos
Clinical Significance of Antibiotic Resistance
Mechanism of Antibiotic Resistance in MRSA
Development of Antibiotic Resistance
Antibiotic Selection
Microbiota of the Urogenital Tract
Antimicrobial Effectiveness

