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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their access...

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Related Experiment Video

Updated: May 13, 2026

Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

Published on: March 31, 2018

Decrease of apoptosis markers during adipogenic differentiation of mesenchymal stem cells from human adipose tissue.

Debora Lo Furno¹, Adriana C E Graziano, Silvia Caggia

¹Department of Bio-medical Sciences, Section of Physiology, University of Catania, V.le A. Doria 6, 95125 Catania, Italy.

Apoptosis : an International Journal on Programmed Cell Death

|March 13, 2013

Summary

This summary is machine-generated.

This study reveals that during the process of converting adipose-derived stem cells into fat cells, key apoptosis markers like p53 and Bax decrease over time. Understanding these changes in apoptotic markers is crucial for tissue repair and regeneration applications.

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Last Updated: May 13, 2026

Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

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Published on: March 31, 2018

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

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Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Published on: April 7, 2023

Area of Science:

Biomedical Sciences
Cell Biology
Regenerative Medicine

Background:

Mesenchymal stem cells (MSCs) from adipose tissue (AT) are vital for tissue repair.
The mechanisms of apoptosis during MSC adipogenic differentiation are not well understood.
Investigating apoptotic markers provides insights into cell fate during differentiation.

Purpose of the Study:

To analyze the expression of apoptotic markers during in vitro adipogenic differentiation of AT-MSCs.
To elucidate the role of the PI3K/AKT signaling pathway in this process.
To provide data for optimizing AT-MSC applications in regenerative medicine.

Main Methods:

Western blot analysis of apoptotic markers (p53, AKT, pAKT, Bax, PDCD4, PTEN) and adipogenic markers (PPAR-γ, FABP).
Immunocytochemistry to confirm MSC identity.
Sudan III staining to assess lipid accumulation and adipocyte differentiation.
Time-course analysis over 28 days of in vitro culture.

Main Results:

Adipogenic differentiation medium successfully induced differentiation, with >50% of MSCs becoming adipocytes within 4 weeks.
Lipid accumulation increased in a time-dependent manner, correlating with PPAR-γ and FABP expression.
The PI3K/AKT signaling pathway was activated, while p53, PTEN, PDCD4, and Bax protein levels decreased over time.

Conclusions:

Adipogenic differentiation of AT-MSCs involves a time-dependent downregulation of specific apoptotic markers.
Activation of the PI3K/AKT pathway is associated with these changes.
Findings contribute to understanding AT-MSC behavior for tissue repair and regeneration.