Test concentration setting for fish in vivo endocrine screening assays
James R Wheeler1, Grace H Panter, Lennart Weltje
1Syngenta Environmental Safety, Jealott's Hill International Research Centre, Bracknell, Berkshire RG42 6EY, United Kingdom. James.Wheeler@Syngenta.com
Chemosphere
|March 14, 2013
Summary
This study proposes optimal test concentrations for fish screening assays to detect endocrine disruptors, aiming to reduce false positives and negatives in regulatory testing.
Area of Science:
- Environmental Toxicology
- Endocrinology
- Aquatic Toxicology
Background:
- Standardized fish in vivo screening methods for endocrine active substances (EAS) targeting the hypothalamic-pituitary-gonadal axis exist (OECD, US-EPA).
- Regulatory testing requires careful selection of test concentrations to ensure accurate assessment of endocrine disruption.
- Systemic toxicity can confound endocrine-specific endpoints, leading to misinterpretation of results.
Keywords:
Concentration settingEndocrineFishMaximum Tolerated ConcentrationScreening assaySystemic toxicity
