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Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
Published on: February 9, 2015
Isolation of mesenchymal stem cells from human dermis
Tsutomu Soma1, Jiro Kishimoto, David Fisher
1Shiseido Innovative Science Research and Development Center, Yokohama, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|March 14, 2013
Summary
Mammalian skin contains multipotent stem cells. This study details methods for isolating mesenchymal stem cells (MSCs) using low-serum conditions and magnetic cell sorting for efficient collection.
Area of Science:
- Cell Biology
- Stem Cell Research
- Dermatology
Background:
- Mammalian dermis harbors multipotent stem cells, including skin-derived precursors (SKPs).
- SKPs exhibit sphere formation in suspension culture.
- Mesenchymal stem cells (MSCs) are characterized as adherent fibroblastic cells.
Purpose of the Study:
- To describe a procedure for isolating mesenchymal stem cells (MSCs).
- To outline a method for collecting MSCs using magnetic cell sorting.
- To differentiate isolation and collection protocols for distinct stem cell types.
Main Methods:
- Isolation of MSCs under low-serum culture conditions.
- Collection of MSCs utilizing magnetic cell sorting (MACS).
- Comparative analysis of SKP and MSC culture characteristics.
Main Results:
- Successful isolation of adherent fibroblastic cells consistent with MSCs.
- Demonstration of efficient MSC collection via magnetic cell sorting.
- Establishment of distinct culture protocols for SKPs and MSCs.
Conclusions:
- Mesenchymal stem cells can be effectively isolated from mammalian dermis.
- Low-serum conditions facilitate MSC isolation.
- Magnetic cell sorting provides a viable method for MSC collection.
