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Stem Cell Culture

Stem Cell Culture

Stem cell research aims to find ways to use stem cells to regenerate and repair cellular damage. Over time, most adult cells undergo the wear and tear of aging and lose their ability to divide and repair themselves. Stem cells do not display a particular morphology or function. Adult stem cells, which exist as a small subset of cells in most tissues, keep dividing and can differentiate into a number of specialized cells generally formed by that tissue. These cells enable the body to renew and...

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously renew...

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Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
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Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own EpiSCs...

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Related Experiment Video

Updated: May 13, 2026

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Published on: March 31, 2011

Skin stem cells.

Keita Inoue¹, Kotaro Yoshimura

¹Department of Plastic and Reconstructive Surgery, Shizuoka Cancer Center, Shizuoka, Japan.

Methods in Molecular Biology (Clifton, N.J.)

|March 14, 2013

Summary

Skin stem cells reside in the epidermis and hair follicle bulge. This study details a method to isolate human hair follicle bulge stem cells using fluorescence-activated cell sorting for further research.

Area of Science:

Dermatology and Stem Cell Biology
Cellular and Molecular Biology

Background:

Skin stem cells are crucial for tissue regeneration and are found in the epidermal basement membrane and hair follicle bulge.
Hair follicle bulge stem cells possess a higher hierarchical stemness compared to epidermal stem cells.

Purpose of the Study:

To describe a standardized method for isolating human hair follicle bulge stem cells.
To enable subsequent functional analyses of these specific stem cells.

Main Methods:

Utilizing fluorescence-activated cell sorting (FACS).
Employing a specific combination of surface antigen labeling for cell identification.
Isolation of the bulge stem cell subset from human hair follicles.

Main Results:

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Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

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A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Published on: August 25, 2018

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Last Updated: May 13, 2026

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Isolation and Culture of Adult Epithelial Stem Cells from Human Skin

Published on: March 31, 2011

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin

Published on: April 29, 2016

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Published on: August 25, 2018

Successful isolation of a distinct bulge stem cell population from human hair follicles.
The described method allows for the enrichment of cells with high stemness potential.

Conclusions:

The developed FACS method provides a reliable approach for isolating human hair follicle bulge stem cells.
This isolation technique is essential for advancing research into skin stem cell biology and regenerative medicine.