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Image processing by microcomputer on ultrastructure of griseofulvin-resistant fungi in favus
Y C Zheng1, Z R Zhu, P J Liang
1Department of Dermatology, Xiehe Hospital, Tongji Medical University, Wuhan.
Abstract:
The ultrastructure of 5 griseofulvin-resistant fungi of favus was studied by image processing with microcomputer. It was found that the cell walls of the fungi consisted of 8 layers, and the inner layer containing cytoplasm was loose. It was also found that all structures within the cytoplasm possessed a 1-3 layers integral envelope with chromatins in the nucleus. These might be contributing factors in the development of resistance to griseofulvin. This multiple-layered, thick cell wall might act as a barrier responsible for the impermeability of the cell to griseofulvin.
Insights
Griseofulvin-resistant fungi exhibit a unique, multi-layered cell wall structure. This thick fungal cell wall may act as a barrier, preventing griseofulvin from entering and causing resistance.
Area of Science:
- Mycology
- Cell Biology
- Antifungal Resistance
Background:
- Favus is a superficial fungal infection.
- Griseofulvin is a common antifungal medication.
- Understanding fungal resistance mechanisms is crucial for effective treatment.
Purpose of the Study:
- To investigate the ultrastructural characteristics of griseofulvin-resistant fungi from favus cases.
- To identify potential cellular features contributing to griseofulvin resistance.
Main Methods:
- Microcomputer-assisted image processing was used to analyze fungal ultrastructure.
- Detailed examination of fungal cell wall and cytoplasmic components.
Main Results:
- Griseofulvin-resistant fungi displayed an 8-layered cell wall.
- The inner cytoplasmic layer was observed to be loose.
- Intracytoplasmic structures, including the nucleus, were enclosed by 1-3 layered envelopes.
Conclusions:
- The multi-layered, thickened cell wall is a potential barrier to griseofulvin.
- These structural adaptations may contribute to the observed griseofulvin resistance in favus fungi.