Graphene bridged enzyme electrodes for glucose biosensing application.

Area of Science:

• Biomaterials Science
• Electrochemistry
• Nanotechnology

Background:

• Enzyme electrodes are crucial for biosensing applications.
• Controlling enzyme immobilization is key to enhancing electrode performance.
• Graphene offers unique properties for electrode fabrication.

Purpose of the Study:

• To develop a novel method for fabricating enzyme electrodes with controlled layer architecture.
• To investigate the self-assembly of pyrene-functionalized glucose oxidase (GOx) onto graphene.
• To evaluate the biocatalytic activity and sensing performance of the fabricated electrodes.

Main Methods:

• Modification of GOx with pyrene functionalities for non-covalent self-assembly.
• Layer-by-layer assembly of graphene and pyrene-functionalized GOx.
• Fabrication of mono- and multi-layered enzyme electrodes.
• Characterization using fluorescence spectroscopy and electrochemical techniques.

Main Results:

• Successful attachment of pyrene to GOx, retaining significant biocatalytic activity (>76%).
• Controlled fabrication of enzyme electrodes with adjustable layers.
• Optimal biocatalytic activity observed with up to four layers.
• Demonstrated reliable application in human serum analysis with high sensitivity and stability.

Conclusions:

• The developed layer-by-layer self-assembly method provides precise control over enzyme electrode nanostructure.
• The fabricated electrodes show promising performance for sensitive and stable glucose detection in biological samples.
• This approach offers a versatile platform for developing advanced biosensors.