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Related Experiment Video

Updated: Jul 14, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

Developing digital PCR technologies based on wettable substrates.

Huimin Wang1,2, Bingli Liu1,2, Feika Bian2

  • 1State Key Laboratory of Targeting Oncology, National Center for International Research of Bio-targeting Theranostics, Guangxi Key Laboratory of Bio-targeting Theranostics, Collaborative Innovation Center for Targeting Tumor Diagnosis and Therapy, Guangxi Talent Highland of Major New Drugs Innovation and Development, Targeting Theranostics Research Center of Guangxi Higher Education, Guangxi Medical University, Nanning, Guangxi, 530021, China. yongxiang_zhao@126.com.

The Analyst
|July 13, 2026
PubMed
Summary

Digital PCR (dPCR) offers absolute nucleic acid quantification using wettable substrates. This review covers dPCR

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Materials Science

Background:

  • Polymerase Chain Reaction (PCR) has evolved significantly since 1985.
  • Digital PCR (dPCR) represents a major advancement, offering absolute quantification.
  • Wettable substrates enhance dPCR with automated droplet manipulation and reduced reagent use.

Purpose of the Study:

  • To review the evolution of PCR technologies, focusing on digital nucleic acid detection.
  • To discuss strategies for engineering wettable substrates for dPCR.
  • To explore the biomedical applications, challenges, and future directions of dPCR.

Main Methods:

  • Historical overview of PCR, qPCR, and dPCR.
  • Analysis of three wettable substrate engineering approaches: surface roughening, modification, and patterning.
  • Review of dPCR applications in biomedicine.

Main Results:

  • Wettable substrate-based dPCR provides automated droplet manipulation, low reagent consumption, and minimal cross-contamination.
  • Key applications in biomedicine are highlighted.
  • Current challenges and future prospects are identified.

Conclusions:

  • dPCR with wettable substrates is a powerful tool for absolute nucleic acid quantification.
  • Future advancements lie in materials innovation, AI-assisted analysis, and portable point-of-care testing (POCT) platforms.
  • Continued development promises broader accessibility and application of dPCR technology.