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Related Experiment Video

Updated: May 13, 2026

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

Published on: April 22, 2011

Role for compartmentalization in nephron progenitor differentiation.

Aaron C Brown¹, Sree Deepthi Muthukrishnan, Justin A Guay

¹Center for Molecular Medicine, Maine Medical Center Research Institute, Scarborough, ME 04074, USA.

Proceedings of the National Academy of Sciences of the United States of America

|March 15, 2013

Summary

This summary is machine-generated.

Bone morphogenetic protein-SMAD signaling drives embryonic nephron progenitor cells from a CITED1+ compartment to a SIX2-only compartment. This transition enables WNT/β-catenin induction, crucial for kidney development.

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Isolation and Culture of Cells from the Nephrogenic Zone of the Embryonic Mouse Kidney

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Guided Differentiation of Mature Kidney Podocytes from Human Induced Pluripotent Stem Cells Under Chemically Defined Conditions

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Published on: July 2, 2020

Area of Science:

Developmental Biology
Renal Physiology
Cell Biology

Background:

Embryonic nephron progenitor cells reside in distinct compartments with poorly understood roles.
Understanding progenitor cell compartmentalization is key to unraveling nephrogenesis.

Purpose of the Study:

To elucidate the functional significance of molecularly distinct compartments in embryonic nephron progenitor cells.
To investigate the role of bone morphogenetic protein-SMAD signaling in progenitor cell fate and WNT/β-catenin responsiveness.

Main Methods:

Analysis of gene expression markers (CITED1, SIX2) in progenitor cell compartments.
Investigating signaling pathway activation (BMP-SMAD, WNT/β-catenin) in relation to cell transitions.

Main Results:

Identified a transition of nephron progenitors from CITED1+ to SIX2-only compartments.
Demonstrated that CITED1+ cells are refractory to WNT/β-catenin signaling, while SIX2-only cells are inducible.
Showed that BMP-SMAD signaling facilitates this transition, enabling WNT/β-catenin responsiveness.

Conclusions:

Proposes a model where BMP-SMAD signaling regulates progenitor cell transition between compartments.
This transition is essential for maintaining undifferentiated progenitors and enabling subsequent WNT/β-catenin-driven epithelialization during nephrogenesis.