Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Amino Acid Biosynthetic Pathways

Amino Acid Biosynthetic Pathways

Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which provide...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Automated Gait Assessment for Rehabilitation Training Using Pose Tracking and Dynamic Time Warping.

Diagnostics (Basel, Switzerland)·2026

Same author

iPSC-based drug discovery identified the Hippo signaling pathway as a therapeutic target in the fibrosis of NPHP1-deficient nephronophthisis.

Stem cell research & therapy·2025

Same author

Identifying optimal puncture position by a real-time image analysis for Piezo-ICSI: a prospective randomized sibling oocyte study.

Reproductive biomedicine online·2024

Same author

Rupture Prediction for Microscopic Oocyte Images of Piezo Intracytoplasmic Sperm Injection by Principal Component Analysis.

Journal of clinical medicine·2022

Same author

Optimal puncture position for ICSI can be detected by image analysis using Local Binary Pattern.

Reproductive biomedicine online·2022

Same author

Singing Experience Influences RSST Scores.

Healthcare (Basel, Switzerland)·2022

Search research articles

Related Experiment Video

Updated: May 13, 2026

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor (LATS) Biosensor

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor (LATS) Biosensor

Published on: September 13, 2018

Okadaic Acid: a tool to study the hippo pathway.

Yutaka Hata¹, Shikshya Timalsina, Sainawaer Maimaiti

¹Department of Medical Biochemistry, Graduate School of Medicine, Tokyo Medical and Dental University, Tokyo 113-8519, Japan. yuhammch@tmd.ac.jp

|March 16, 2013

Summary

Okadaic acid activates the Hippo pathway by enhancing MST1/MST2 kinase phosphorylation, a key tumor suppressor pathway. Researchers should consider other phosphatase targets when using okadaic acid to study the Hippo pathway.

More Related Videos

Culturing and Manipulation of O9-1 Neural Crest Cells

Culturing and Manipulation of O9-1 Neural Crest Cells

Published on: October 9, 2018

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

Related Experiment Videos

Last Updated: May 13, 2026

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor (LATS) Biosensor

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor (LATS) Biosensor

Published on: September 13, 2018

Culturing and Manipulation of O9-1 Neural Crest Cells

Culturing and Manipulation of O9-1 Neural Crest Cells

Published on: October 9, 2018

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

Published on: March 27, 2020

Area of Science:

Cell biology
Molecular signaling
Cancer research

Background:

The Hippo pathway is a critical tumor suppressor signaling network.
Mammalian Ste20-like kinases (MST1 and MST2) are core kinases regulating the Hippo pathway.
Protein phosphatase 2A (PP2A) negatively regulates MST1/MST2 activity via dephosphorylation.

Purpose of the Study:

To review the molecular architecture of the Hippo pathway.
To explain the regulation of MST kinases by PP2A.
To discuss the impact of okadaic acid on Hippo pathway components.

Main Methods:

Review of existing literature on the Hippo pathway.
Analysis of MST kinase regulation by PP2A.
Discussion of okadaic acid's effects on Hippo pathway signaling.

Main Results:

Okadaic acid enhances MST1 and MST2 phosphorylation, activating the Hippo pathway.
MST1 and MST2 activities are regulated by autophosphorylation and PP2A.
Other Hippo pathway components may also be targets of okadaic acid-sensitive phosphatases.

Conclusions:

Okadaic acid is a tool to activate the Hippo pathway, but its effects may extend beyond MST kinases.
Careful consideration is needed when using okadaic acid to study the Hippo pathway.
Identifying additional phosphatase targets is crucial for understanding Hippo pathway regulation.