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Yeast proteome variations reveal different adaptive responses to grape must fermentation
Mélisande Blein-Nicolas1, Warren Albertin, Benoît Valot
1INRA, PAPPSO, UMR 0320/8120 Génétique Végétale, Gif sur Yvette, France.
Molecular Biology and Evolution
|March 16, 2013
Summary
Saccharomyces cerevisiae and Saccharomyces uvarum yeast species evolved different proteomes and metabolic pathways, yet both achieve grape must fermentation. This highlights distinct evolutionary routes for this key industrial trait.
Area of Science:
- * Evolutionary biology
- * Microbial genomics
- * Proteomics
Background:
- * Saccharomyces cerevisiae and Saccharomyces uvarum diverged ~100 million years ago within the Saccharomyces sensu stricto clade.
- * Both species possess duplicated genes for glucose fermentation and can ferment grape must.
- * Despite shared traits, they exhibit distinct evolutionary histories and phenotypic differences.
Purpose of the Study:
- * To investigate how the evolutionary divergence of S. cerevisiae and S. uvarum is reflected in their proteomes.
- * To analyze proteomic variability and its correlation with phenotypic traits and genetic differences.
- * To understand the evolutionary mechanisms underlying grape must fermentation in these two yeast species.
Main Methods:
- * Quantitative mass spectrometry was employed to analyze the proteomes of domesticated strains.
- * Genetic variability was assessed across strains.
- * Proteomic data was compared with phenotypic and genetic variation data.
Main Results:
- * Massive variations in protein abundances were observed, clearly differentiating S. cerevisiae and S. uvarum.
- * Abundance differences in metabolic pathways correlated with known discriminatory phenotypic traits.
- * Proteins encoded by duplicated genes were differentially recruited between the species.
- * Some S. uvarum strains and certain S. cerevisiae strains showed similar fermentation performance despite significant proteomic and genomic disparities.
Conclusions:
- * The capacity for grape must fermentation in S. cerevisiae and S. uvarum evolved through independent evolutionary pathways.
- * These distinct evolutionary routes involve different metabolic pathways and differential utilization of duplicated genes.
- * Proteomic and genomic differences do not always predict phenotypic similarity in fermentative performance.
Keywords:
adaptive evolutiongenetic diversitygenome duplicationglucose fermentationshotgun proteomicsMore Related Videos
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