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Related Experiment Video

Updated: May 13, 2026

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors
16:19

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Published on: September 10, 2013

pH-Sensitive MR Responses Induced by Dendron-Functionalized SPIONs.

Indrajit Saha1, Kathleen E Chaffee, Chuansong Duanmu

  • 1Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, IL 62901.

The Journal of Physical Chemistry. C, Nanomaterials and Interfaces
|March 16, 2013
PubMed
Summary
This summary is machine-generated.

Surface-functionalized superparamagnetic iron oxide nanoparticles (SPIONs) show pH-sensitive magnetic resonance responses. Melamine-dendron SPIONs exhibit tunable relaxivity and stability, enabling pH-responsive imaging.

Keywords:
MRINMRcontrast agentdendrimeriron oxidesuperparamagnetic

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Nanotechnology

Background:

  • Superparamagnetic iron oxide nanoparticles (SPIONs) are widely used in biomedical imaging.
  • Developing SPIONs with tailored responses to physiological parameters like pH is crucial for advanced diagnostics.

Purpose of the Study:

  • To investigate the pH-sensitive magnetic resonance (MR) properties of surface-functionalized SPIONs.
  • To optimize melamine-dendron functionalization for enhanced MR relaxivity and stability.
  • To explore the potential of these SPIONs as pH-responsive contrast agents.

Main Methods:

  • Synthesis and characterization of SPIONs functionalized with different generations of melamine-dendrons.
  • Measurement of molar relaxivities (R2m, R1m) at varying pH and ionic strengths.
  • Transmission electron microscopy (TEM) to study SPION aggregation.
  • Cellular uptake studies in HeLa cell cultures.

Main Results:

  • Optimized SPIONs achieved high molar relaxivities (e.g., R2m ~300 mM⁻¹·s⁻¹ at 7 T) and stability.
  • Significant pH sensitivity was observed near the pKa of melamine, with effects increasing with dendron generation.
  • pH-dependent SPION clustering, modulated by surface charge and ionic strength, was identified as the mechanism for MR response.
  • Ratiometric MR signals were achievable by exploiting opposing R2m and R2m* trends.

Conclusions:

  • Melamine-dendron functionalized SPIONs offer tunable, pH-sensitive MR properties.
  • These nanoparticles show promise as responsive contrast agents for pH imaging in biological systems.
  • The MR response is controllable through rational design of surface chemistry.