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Related Concept Videos

Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA (thiogalactoside...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...

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Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
08:51

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling

Published on: June 25, 2015

Extrachromosomal inducible expression.

Douwe M Veltman1, Peter J M Van Haastert

  • 1Beatson Institute for Cancer Research, Glasgow, UK. d.veltman@beatson.gla.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|March 16, 2013
PubMed
Summary
This summary is machine-generated.

This study details a doxycycline-inducible expression system for controlled protein production. It offers two plasmid types for gene expression regulation, crucial for toxic proteins or those causing side effects.

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Gene Expression Systems

Background:

  • Inducible expression systems are vital for managing proteins with adverse effects.
  • Controlled protein expression is essential for toxic proteins and developmental studies.

Purpose of the Study:

  • To describe a doxycycline-inducible expression system for precise control.
  • To present protocols for plasmid construction and inducible protein expression.

Main Methods:

  • Development of two doxycycline-inducible plasmid types.
  • Optimization of gene expression control using doxycycline.
  • Detailed protocols for plasmid construction and protein induction.

Main Results:

  • Successful implementation of a doxycycline-regulated gene expression system.
  • Demonstration of inducible expression via plasmid construction.
  • Provision of practical protocols for researchers.

Conclusions:

  • The doxycycline-inducible system enables controlled expression of proteins.
  • This system is suitable for expressing toxic proteins or those affecting growth.
  • The provided protocols facilitate the use of this advanced expression technology.