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Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching
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Characterization of one- and two-photon photochemical uncaging efficiency

Alexandre Specht1, Frederic Bolze, Jean Francois Nicoud

  • 1Department of Bioorganic Chemistry, Centre National de la Recherche Scientifique, Université Louis Pasteur Strasbourg, Illkirch, France.

Methods in Molecular Biology (Clifton, N.J.)
|March 16, 2013
PubMed
Summary

This study details methods for characterizing light-activated uncaging reactions. It focuses on measuring one-photon quantum yields and two-photon cross-sections for photoremovable protecting groups.

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