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13:32
High Content Screening in Neurodegenerative Diseases
Published on: January 6, 2012
The industrialisation of cellular screening.
1GlaxoSmithKline, Screening and Compound Profiling Department, New Frontiers Science Park, Third Avenue, Harlow, Essex, CM19 5AW, UK +44 1279 622661 ; +44 1279 6278781 ; steve.x.rees@gsk.com.
Expert Opinion on Drug Discovery
|March 19, 2013
Summary
Automated cell culture and cryopreserved cells enhance consistency in cell-based screening. Implementing manufacturing best practices and automation improves assay performance for drug discovery and clinical candidate development.
Area of Science:
- Pharmacology and Drug Discovery
- Cell Biology and Assay Development
Background:
- Cell-based assays are the preferred method for compound screening in drug discovery.
- These assays are crucial for identifying lead compounds and supporting the development of clinical candidates.
Purpose of the Study:
- To describe technologies and working practices for improving cell-based assay consistency.
- To detail methods for reducing the costs associated with cell-based screening.
- To highlight the application of these improvements in an industrial drug discovery setting.
Main Methods:
- Adoption of automated cell culture or use of cryopreserved cells to minimize biological variability.
- Implementation of simple assay protocols and automation solutions for enhanced assay performance.
- Integration of manufacturing industry working practices to reduce process variability.
Main Results:
- Improved consistency and reduced costs in cell-based screening processes.
- Enhanced assay performance through automation and streamlined protocols.
- Successful application of these strategies in an industrial environment.
Conclusions:
- Automated methods and cryopreserved cells are key to consistent cell-based screening.
- Simple protocols and automation solutions significantly improve assay performance.
- Adoption of these technologies and practices enables effective identification and optimization of clinical candidates.
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