Myelin basic protein immunosensor for multiple sclerosis detection based upon label-free electrochemical impedance...

Area of Science:

• Electrochemistry
• Biosensors
• Immunosensors

Background:

• Multiple Sclerosis (MS) diagnosis can be aided by detecting autoantibodies like Anti-Myelin Basic Protein (Anti-MBP).
• Current detection methods may lack the sensitivity or speed required for timely diagnosis.
• Development of novel biosensors is crucial for improved MS diagnostics.

Purpose of the Study:

• To develop a highly sensitive impedimetric immunosensor for the determination of Anti-Myelin Basic Protein (Anti-MBP).
• To immobilize Myelin Basic Protein (MBP) on modified platinum electrodes for enhanced electron transfer.
• To evaluate the immunosensor's performance in detecting Anti-MBP in human serum and cerebrospinal fluid (CSF).

Main Methods:

• Immobilization of MBP onto Gelatin and Gelatin-Titanium Dioxide (TiO₂) modified platinum electrodes.
• Electrochemical characterization using Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS).
• Determination of Anti-MBP using label-free impedimetric measurements and calibration curves.

Main Results:

• The Gelatin-TiO₂-MBP electrode exhibited a more reversible electron transfer reaction.
• The immunosensors demonstrated high sensitivity with low detection limits (0.1528 ng/ml and 0.1495 ng/ml).
• Rapid response times (46-58 seconds) and specific detection of Anti-MBP in human CSF and serum samples were achieved.

Conclusions:

• A novel, highly sensitive, and rapid impedimetric immunosensor for Anti-MBP detection was successfully developed.
• The Gelatin-TiO₂-MBP modified electrode offers improved electrochemical performance.
• This label-free immunosensor presents a promising tool for the specific determination of Anti-MBP in clinical diagnostics for MS.