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Updated: May 13, 2026

A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators
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A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

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The second extracellular loop of GPCRs determines subtype-selectivity and controls efficacy as evidenced by loop

Benjamin F Seibt1, Anke C Schiedel, Dominik Thimm

  • 1PharmaCenter Bonn, Pharmaceutical Institute, Pharmaceutical Chemistry I, University of Bonn, 53121 Bonn, Germany.

Biochemical Pharmacology
|March 19, 2013
PubMed

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