Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

946
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
946

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-World Outcomes of Prophylaxis With rIX-FP in Germany: A Prospective, Non-Interventional Study in Haemophilia B.

Haemophilia : the official journal of the World Federation of Hemophilia·2026
Same author

Base editing-derived models of human WDR34 and WDR60 disease alleles replicate retrograde intraflagellar transport (IFT) and hedgehog signaling defects.

Communications biology·2026
Same author

VKORC1L1-mediated vitamin K recycling counters ferroptosis to promote endothelial repair.

Scientific reports·2026
Same author

Psychedelics and Entactogens.

Journal of medicinal chemistry·2026
Same author

Management of Breakthrough Bleeding During Emicizumab Prophylaxis in Acquired Haemophilia A: Data From the GTH-AHA-EMI Study.

Haemophilia : the official journal of the World Federation of Hemophilia·2026
Same author

Neuroglial P2Y<sub>1</sub> receptor signalling differentially contributes to inflammatory neurodegeneration.

Journal of neuroinflammation·2026

Related Experiment Video

Updated: Jun 2, 2025

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1&#946; in Human Monocyte-derived Dendritic Cells
09:04

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

20.8K

Lipopolysaccharide-Neutralizing Peptide Modulates P2X7 Receptor-Mediated Interleukin-1β Release.

Jonas Engelhardt1, Anna Klawonn1, Ann-Kathrin Dobbelstein1

  • 1Pharmaceutical Institute, Pharmacology and Toxicology, University of Bonn, Gerhard-Domagk-Str. 3, 53121 Bonn, Germany.

ACS Pharmacology & Translational Science
|January 16, 2025
PubMed
Summary

Synthetic LPS-neutralizing peptides like Pep19-2.5 show potential for treating inflammatory diseases by modulating P2X receptors. This study reveals Pep19-2.5

More Related Videos

A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide
08:33

A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide

Published on: December 15, 2014

11.7K
Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

11.3K

Related Experiment Videos

Last Updated: Jun 2, 2025

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1&#946; in Human Monocyte-derived Dendritic Cells
09:04

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

20.8K
A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide
08:33

A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide

Published on: December 15, 2014

11.7K
Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
11:48

Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes

Published on: May 31, 2018

11.3K

Area of Science:

  • Immunology
  • Pharmacology
  • Cell Biology

Background:

  • Lipopolysaccharide (LPS)-neutralizing peptides are investigated for sepsis and skin infection therapies.
  • Purinergic ligand-gated ion channels (P2X receptors) are crucial in inflammatory processes.
  • Modulation of P2X receptors is a significant focus in drug development.

Purpose of the Study:

  • To investigate the effects of the synthetic LPS-neutralizing peptide Pep19-2.5 on human P2X receptors.
  • To explore Pep19-2.5's impact on innate immune cells.
  • To determine the therapeutic potential of Pep19-2.5 in inflammatory conditions.

Main Methods:

  • Assessed Pep19-2.5's effect on Ca2+ influx, IL-1β, and LDH release in human macrophages and monocytes.
  • Utilized confocal microscopy to confirm peptide-receptor colocalization.
  • Examined Pep19-2.5's activity on various P2X receptor subtypes in transfected astrocytoma cells.

Main Results:

  • Pep19-2.5 triggered concentration-dependent Ca2+ influx, IL-1β, and LDH release in stimulated immune cells.
  • P2X7 receptors were identified as key mediators of these cellular responses.
  • Pep19-2.5 demonstrated potent modulation of P2X7 and P2X4 receptors, with significant activity on P2X1 and P2X3, and no activity on P2X2.

Conclusions:

  • LPS-neutralizing peptides can act as modulators of P2X receptors.
  • Pep19-2.5's interaction with P2X receptors suggests broader therapeutic applications beyond sepsis.
  • These findings open avenues for developing novel treatments for inflammatory diseases.