Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Outer Layers of the Cell Envelope01:18

Outer Layers of the Cell Envelope

The outermost layers of prokaryotic cells play a critical role in their survival, virulence, and interaction with the environment. These layers, often composed of polysaccharides, polypeptides, or proteins, form protective and adhesive structures that vary in organization and function.Capsules and Slime LayersCapsules are highly organized, tightly bound layers that firmly attach to the bacterial cell wall. Capsules are usually made of polysaccharides, though some are made of polypeptides. These...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

S-layers as natural building blocks for nanobiotechnology and synthetic biology.

Current opinion in microbiology·2026
Same author

S-layers: from a serendipitous discovery to a toolkit for nanobiotechnology.

Quarterly reviews of biophysics·2025
Same author

SymProFold: Structural prediction of symmetrical biological assemblies.

Nature communications·2024
Same author

Exploring surface structures.

eLife·2024
Same author

Activity of Single Insect Olfactory Receptors Triggered by Airborne Compounds Recorded in Self-Assembled Tethered Lipid Bilayer Nanoarchitectures.

ACS applied materials & interfaces·2023
Same author

Scalable biomimetic sensing system with membrane receptor dual-monolayer probe and graphene transistor arrays.

Science advances·2023
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 13, 2026

Au-Interaction of Slp1 Polymers and Monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as Tools for Biomolecule-metal Studies
08:29

Au-Interaction of Slp1 Polymers and Monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as Tools for Biomolecule-metal Studies

Published on: January 19, 2016

Nanotechnology with s-layer proteins.

Bernhard Schuster1, Uwe B Sleytr

  • 1Department of NanoBiotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.

Methods in Molecular Biology (Clifton, N.J.)
|March 19, 2013
PubMed
Summary
This summary is machine-generated.

Nanosciences leverage self-assembly systems for innovation. Bacterial S-layer proteins enable precise construction of nanostructures for bioanalytical sensors and molecular electronics.

More Related Videos

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly
16:33

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

Published on: April 17, 2014

Related Experiment Videos

Last Updated: May 13, 2026

Au-Interaction of Slp1 Polymers and Monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as Tools for Biomolecule-metal Studies
08:29

Au-Interaction of Slp1 Polymers and Monolayer from Lysinibacillus sphaericus JG-B53 - QCM-D, ICP-MS and AFM as Tools for Biomolecule-metal Studies

Published on: January 19, 2016

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly
16:33

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

Published on: April 17, 2014

Area of Science:

  • Nanosciences
  • Supramolecular chemistry
  • Biomaterials

Background:

  • Nanosciences integrate diverse fields like biology and material sciences.
  • Self-assembly systems offer a pathway to create reproducible supramolecular aggregates.
  • Bacterial S-layer proteins are identified as key building blocks for nanostructure fabrication.

Purpose of the Study:

  • To explore the use of S-layer proteins for innovative supramolecular assembly.
  • To develop methods for creating nanostructures with dimensions from nanometers to tens of nanometers.
  • To enable controlled immobilization and confinement of biomolecules for advanced applications.

Main Methods:

  • Utilizing crystalline bacterial cell-surface proteins (S-layer proteins) as molecular building blocks.
  • Employing self-assembly principles for spontaneous molecular association.
  • Developing techniques for ordered immobilization of biomolecules on solid substrates.

Main Results:

  • Demonstrated innovative approaches for assembling supramolecular structures using S-layer proteins.
  • Successfully created functional units with dimensions in the nanometer range.
  • Established S-layer proteins as versatile components in a molecular construction kit.

Conclusions:

  • S-layer proteins provide a powerful platform for nanostructure fabrication.
  • Controlled biomolecule immobilization is crucial for nanodevice development.
  • These nanostructures have potential applications in bioanalytical sensors, biochips, and molecular electronics.