Related Experiment Video
Updated: May 13, 2026

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
Published on: July 29, 2015
Reprogramming of adult human neural stem cells into induced pluripotent stem cells
Li-Qian Xie1, Hua-Ping Sun, Tian Wang
1Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200040, China.
Background:
Since an effective method for generating induced pluripotent stem cells (iPSCs) from human neural stem cells (hNSCs) can offer us a promising tool for studying brain diseases, here we reported direct reprogramming of adult hNSCs into iPSCs by retroviral transduction of four defined factors.
Methods:
NSCs were successfully isolated and cultured from the hippocampus tissue of epilepsy patients. When combined with four factors (OCT3/4, SOX2, KLF4, and c-MYC), iPSCs colonies were successfully obtained.
Results:
Morphological characterization and specific genetic expression confirmed that these hNSCs-derived iPSCs showed embryonic stem cells-like properties, which include the ability to differentiate into all three germ layers both in vitro and in vivo.
Conclusion:
Our method would be useful for generating human iPSCs from NSCs and provide an important tool for studying neurological diseases.
More Related Videos
10:52Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
Published on: January 19, 2020
09:36Lineage-reprogramming of Pericyte-derived Cells of the Adult Human Brain into Induced Neurons
Published on: May 12, 2014
Related Concept Videos
Induced Pluripotent Stem Cells
Induced Pluripotent Stem Cells
Somatic cells are...
Induced Pluripotent Stem Cells
Somatic to iPS Cell Reprogramming
iPS Cell Differentiation
Stem Cell Culture