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Updated: May 13, 2026

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension
Published on: September 11, 2020
Flow field-flow fractionation: critical overview
1Unit for Analysis and Synthesis, Department of Chemistry, Center for Chemistry and Chemical Engineering, Faculty of Engineering LTH, Lund University, Box 124, SE-221 00 Lund, Sweden. Karl-Gustav.Wahlund@analykem.lu.se
Abstract:
This overview regards some critical issues in performing flow field-flow fractionation (flow FFF, FlFFF, AF4, HF-FlFFF, HF5). It includes the channel thickness, void time, channel-flow parabolic profile, channel-flow velocity gradient, uniformity of the cross-flow, sample injection time, relaxation/focusing time, width of sample starting zone, retention level, theoretical and experimental zone broadening, hydrodynamic threshold immobilisation/re-mobilisation, sample loss and adsorption, membrane fouling, sample mass overloading, problems with symmetrical channels, non-spherical sample particles, and method development. Good method development practice (GMDP) and good fractogram practice (GFP) is suggested.
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