[Determination of copper, magnesium and zinc in mononuclear leukocytes by flame atomic absorption spectrometry]
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Updated: May 13, 2026

Atomic Absorbance Spectroscopy to Measure Intracellular Zinc Pools in Mammalian Cells
Published on: May 16, 2019
Kareem Rahn-Chique1, Nereida Carrión, Miguel Murillo
1Centro de Química Analítica, Escuela de Química, Facultad de Ciencias, Universidad Central de Venezuela, Caracas, Venezuela. krahn@ivic.gob.ve
Abstract:
In this paper we evaluated a new micro-flow injector for the determination of the concentrations of Cu, Mg and Zn in mononuclear blood cells. This device analyzed sample volumes in the order of microliters by flame atomic absorption spectrometry; it is inexpensive, and easy to build and to adapt to the conventional injector of the atomic absorption spectrophotometer. Detection limits of 106, 65 and 37 microg L(-1) for Cu, Mg and Zn were obtained, respectively. The percentages of recovery tests were found between 98 and 110%.
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