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Updated: May 13, 2026

HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
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Protoanemonin content variation between Clematis spp.: leaf, stem and root.

Fangming Jin1, Christian Narkowicz, Glenn A Jacobson

  • 1School of Pharmacy, University of Tasmania, Private Bag 26, Hobart, Tasmania 7001, Australia.

Natural Product Communications
|March 22, 2013
PubMed
Summary
This summary is machine-generated.

Protoanemonin concentrations in Clematis species vary by plant part, with leaves showing the highest levels. This study is the first to quantify protoanemonin variation across different Clematis plant tissues.

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HPLC Coupled with Chemical Fingerprinting for Multi-Pattern Recognition for Identifying the Authenticity of Clematidis Armandii Caulis
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Published on: March 6, 2019

Area of Science:

  • Phytochemistry
  • Pharmacognosy
  • Plant Science

Background:

  • Clematis species are known for containing biologically active compounds.
  • Protoanemonin is a significant constituent found in various Clematis species.
  • Understanding the distribution of active compounds is crucial for medicinal applications.

Purpose of the Study:

  • To quantify protoanemonin content in different parts (leaf, stem, root) of selected Clematis species.
  • To investigate variations in protoanemonin concentration among different Clematis taxa.
  • To establish a baseline for protoanemonin distribution within Clematis plants.

Main Methods:

  • Gas Chromatography-Mass Spectrometry (GC-MS) was employed for quantitative analysis.
  • Extraction of protoanemonin was performed on leaf, stem, and root samples.
  • Samples were analyzed from one Chinese and four Australian Clematis taxa.

Main Results:

  • Protoanemonin concentrations differed significantly across plant tissues and species.
  • Leaves consistently exhibited higher protoanemonin concentrations compared to stems and roots.
  • This research provides the first detailed analysis of protoanemonin variation in Clematis spp. tissues.

Conclusions:

  • Plant part significantly influences protoanemonin accumulation in Clematis.
  • Leaf tissues represent a primary source of protoanemonin within these species.
  • Findings contribute to the chemotaxonomic understanding and potential utilization of Clematis species.