Related Experiment Video
Updated: May 13, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Fully validated simultaneous determination of bisoprolol fumarate and hydrochlorothiazide in their dosage forms using
Burcin Bozal1, Mehmet Gumustas, Burcu Dogan-Topal
1Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, Tandogan, Ankara, Turkey.
Abstract:
Voltammetric, chromatographic, and spectrophotometric methods were developed for the simultaneous determination of bisoprolol fumarate (BIS) and hydrochlorothiazide (HCZ). Differential pulse and square wave voltammetry techniques were used to analyze BIS and HCZ simultaneously by measuring at about 1400 and 1100 mV, respectively. RP-HPLC was the second method for simultaneous analysis of the compounds. The mixture of BIS, HCZ, and moxifloxacin as an internal standard was separated on an RP Zorbax Eclipse XDB-C18 column (150 x 4.6 mm, id, 5 microm particle size) using acetonitrile-15 mM phosphate (25+75, v/v) mobile phase at a 1.0 mL/min flow rate. The third method was based on first derivative of the ratio-spectra method obtained from the measurements of the amplitudes at 246 and 257 nm for BIS and HCZ, respectively. All the proposed methods were effectively applied for the simultaneous determination of BIS and HCZ in tablet dosage forms without any time-consuming extraction, sample preparation, or derivatization procedures.
Related Concept Videos
Bioequivalence studies: Biowaivers
Measurement of Bioavailability: Pharmacodynamic Methods
Therapeutic Drug Monitoring: Drug Analysis Methods
Drug Dissolution: Requirements and Profile Comparison
Bioequivalence of Drugs: Drugs with Multiple Indications
Determination of Multiple Dosing Parameters: Loading and Maintenance Doses
