Related Experiment Video
Updated: May 13, 2026

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation
Published on: February 8, 2015
Internalization of Locked Nucleic Acids/DNA Hybrid Oligomers into Escherichia coli
German M Traglia1, Carol Davies Sala, Juan I Fuxman Bass
1Institute of Microbiology and Medical Parasitology, National Scientific and Technical Research Council (CONICET), University of Buenos Aires , Buenos Aires, Argentina .
Abstract:
Delivery inside the cells is essential for practical application of antisense technologies. The hybrid locked nucleic acid (LNA)/DNA CAAGTACTGTTCCACCA (LNA residues are underlined) was labeled by conjugation to Alexa Fluor 488 (fLNA/DNA) and tested to determine its ability to penetrate Escherichia coli cells and reach the cytoplasm. Flow cytometry analysis showed that the fLNA/DNA was associated with 14% of cells from a stationary phase culture, while association with a labeled isosequential oligodeoxynucleotide was negligible. Laser scanning confocal microscopy confirmed that the fLNA/DNA was located inside the cytoplasm.
Related Concept Videos
Bacterial Transformation
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...
Transformation

