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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...

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Related Experiment Video

Updated: May 13, 2026

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
11:22

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay

Published on: August 26, 2018

Alternative splicing in ascomycetes.

Frank Kempken1

  • 1Abteilung für Botanische Genetik und Molekularbiologie, Botanisches Institut und Botanischer Garten, Christian-Albrechts-Universität zu Kiel, Olshausenstr. 40, 24098 Kiel, Germany. fkempken@bot.uni-kiel.de

Applied Microbiology and Biotechnology
|March 22, 2013
PubMed
Summary
This summary is machine-generated.

Alternative splicing, a key gene expression process, varies across eukaryotes. This review examines alternative splicing in ascomycetes, noting lower rates in yeast-like species compared to filamentous fungi.

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Last Updated: May 13, 2026

Quantitative Analysis of Alternative Pre-mRNA Splicing in Mouse Brain Sections Using RNA In Situ Hybridization Assay
11:22

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Published on: August 26, 2018

Using the E1A Minigene Tool to Study mRNA Splicing Changes
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Area of Science:

  • Molecular Biology
  • Genetics
  • Eukaryotic Gene Expression

Background:

  • Alternative splicing generates diverse messenger RNA (mRNA) molecules from a single gene, impacting protein diversity.
  • The extent and types of alternative splicing differ significantly across eukaryotic organisms.
  • Ascomycetes exhibit generally lower levels of alternative splicing compared to mammals.

Purpose of the Study:

  • To review alternative splicing patterns in ascomycetes.
  • To compare alternative splicing in yeast-like and filamentous fungi.
  • To explore regulatory mechanisms, including riboswitch RNA and protein factors.

Main Methods:

  • Genomic scale analysis of alternative splicing.
  • Case studies from individual research.
  • Survey of alternative splicing in Neurospora crassa.
  • Investigation of riboswitch RNA-mediated regulation.

Main Results:

  • Ascomycetes display a lower extent of alternative splicing than mammals.
  • Yeast-like species have reduced alternative splicing due to fewer introns compared to filamentous fungi.
  • Specific examples and genomic data illustrate alternative splicing in Neurospora crassa.

Conclusions:

  • Alternative splicing is a fundamental yet variable eukaryotic process.
  • Ascomycetes present a distinct model for studying alternative splicing evolution and regulation.
  • Riboswitch RNA and protein factors are implicated in regulating alternative splicing.