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Extraction of ginsenosides from fresh ginseng roots (Panax ginseng C.A. Meyer) using commercial enzymes and high
Hoon H Sunwoo1, Chong-Tai Kim, Do-Yeon Kim
1Faculty of Pharmacy and Pharmaceutical Science, 3-142G Katz Group Centre for Pharmacy & Health Research, University of Alberta, Edmonton, AB, Canada. hsunwoo@ualberta.ca
Abstract:
A combination of high hydrostatic pressure (HHP) and enzymatic hydrolysis (HHP-EH) was applied for the extraction of ginsenosides from fresh ginseng roots (Panax ginseng C.A. Myer). The highest yield of ginsenosides was obtained by using a mixture of three enzymes (Celluclast + Termamyl + Viscozyme) along with HHP (100 MPa, at 50 °C for 12 h) in comparison to control samples (no enzymes, atmosphere pressure, P < 0.05). Total ginsenosides increased by 184% while Rg1 + Rb1 increased by 273%. Application of these conditions significantly increased total ginsenosides by 49% and Rg1 + Rb1 by 103% compared to HHP treatment alone (P < 0.05). The effect of HHP on increased yield of ginsenosides is likely due in part, to acceleration of enzyme activity. Thus HHP-EH significantly improves the extraction of ginsenosides from fresh ginseng roots.
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