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Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies
Published on: August 20, 2021
Draft Genome Sequence of Microbacterium sp. Strain UCD-TDU (Phylum Actinobacteria)
Zachary A Bendiks1, Jenna M Lang, Aaron E Darling
1University of California at Davis, Genome Center, Davis, California, USA.
Researchers sequenced the genome of Microbacterium sp. strain UCD-TDU, an Actinobacteria microbe found in a residential toilet. This draft genome provides a reference for studying bacteria in the built environment.
Area of Science:
- Microbiology
- Genomics
- Actinobacteria
Background:
- Microbes inhabit diverse environments, including the built environment.
- Understanding microbial diversity is crucial for various scientific fields.
- Actinobacteria are a significant phylum with diverse metabolic capabilities.
Purpose of the Study:
- To present the draft genome sequence of Microbacterium sp. strain UCD-TDU.
- To provide a reference genome for microbes isolated from the built environment.
- To support undergraduate research in microbial genomics.
Main Methods:
- Isolation of Microbacterium sp. strain UCD-TDU from a residential toilet.
- Whole-genome sequencing and assembly.
- Bioinformatic analysis of the draft genome.
Main Results:
- The draft genome assembly of Microbacterium sp. strain UCD-TDU consists of 3,746,321 base pairs distributed across 8 scaffolds.
- The genome sequence provides a valuable resource for the Actinobacteria phylum.
- This represents a successful integration of student research with genome sequencing.
Conclusions:
- The availability of the Microbacterium sp. strain UCD-TDU genome sequence enhances our understanding of microbial life in domestic settings.
- This work contributes to the growing database of reference genomes from the built environment.
- It highlights the potential for citizen science and undergraduate research to contribute to genomic studies.
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