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Related Experiment Videos

ATP and ADP in human pre-embryos.

H Slotte1, O Gustafson, L Nylund

  • 1Department of Clinical Chemistry, Karolinska Institutet, Huddinge University Hospital, Sweden.

Human Reproduction (Oxford, England)
|April 1, 1990
PubMed
Summary

Human oocytes and pre-embryos have good energy status, indicated by high ATP/ADP ratios. This finding suggests the luciferin-luciferase assay can assess embryo viability in in-vitro fertilization (IVF) programs.

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Area of Science:

  • Reproductive biology
  • Biochemistry

Background:

  • Assessing the energetic status of human oocytes and pre-embryos is crucial for in-vitro fertilization (IVF) success.
  • Adenosine triphosphate (ATP) and adenosine diphosphate (ADP) levels are key indicators of cellular energy and viability.

Purpose of the Study:

  • To measure ATP and ADP content in spare human oocytes and pre-embryos at various developmental stages.
  • To evaluate the utility of the luciferin-luciferase assay for assessing pre-embryo energy status in IVF.

Main Methods:

  • Utilized a bioluminescence assay based on the firefly luciferin-luciferase reaction.
  • Individually analyzed ATP and ADP content in oocytes and pre-embryos from an IVF program.

Main Results:

  • Oocytes and pre-embryos exhibited low ADP levels across all developmental stages.
  • Elevated ATP/ADP ratios (19-92) were observed, indicating a robust cellular energy status.
  • ATP content varied slightly across stages, with a notable increase in the 6-8 cell stage.

Conclusions:

  • The luciferin-luciferase assay is a sensitive method for evaluating the energy status of human oocytes and pre-embryos.
  • This assay can be a valuable tool for optimizing incubation conditions in human IVF programs.
  • The consistently high ATP/ADP ratios suggest good inherent energy reserves in human gametes and early embryos.

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