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Updated: May 13, 2026

Isolation of Mouse Megakaryocyte Progenitors
10:30

Isolation of Mouse Megakaryocyte Progenitors

Published on: May 20, 2021

Megakaryocyte polyploidy is inhibited by lysyl oxidase propeptide

Alexia Eliades1, Nikolaos Papadantonakis, Shinobu Matsuura

  • 1Department of Biochemistry, Whitaker Cardiovascular Institute, Boston University School of Medicine, Boston, MA USA.

Cell Cycle (Georgetown, Tex.)
|March 23, 2013
PubMed

Insights

Lysyl oxidase propeptide (LOX-PP) reduces megakaryocyte (MK) ploidy and size by impacting cell cycle proteins and microtubules. This suggests LOX-PP may regulate MK polyploidization.

Area of Science:

  • Hematology
  • Cell Biology
  • Biochemistry

Background:

  • Megakaryocytes (MKs) are platelet precursors undergoing endomitosis to achieve polyploidy.
  • Lysyl oxidase (LOX) and its propeptide (LOX-PP) are involved in MK lineage expansion and ploidy.
  • LOX expression is inversely correlated with MK polyploidy.

Purpose of the Study:

  • To investigate the effect of LOX-PP on the ploidy and number of primary MKs.
  • To elucidate the molecular mechanisms by which LOX-PP influences MK endomitosis.

Main Methods:

  • Treatment of mouse bone marrow MKs with LOX-PP.
  • Analysis of MK ploidy, size, and number.
  • Western blotting for ERK1/2 phosphorylation and cell cycle proteins (cyclin D3, cyclin E).
  • Pull-down assays and immunochemistry for LOX-PP interaction with α-tubulin and microtubules.

Main Results:

  • LOX-PP significantly decreased MK ploidy and size but did not affect MK number.
  • LOX-PP treatment reduced ERK1/2 phosphorylation and levels of cyclin D3 and cyclin E.
  • LOX-PP was found to interact with α-tubulin and microtubules.

Conclusions:

  • LOX-PP plays a role in reducing MK ploidy.
  • The interaction of LOX-PP with microtubules and its effect on cell cycle regulators contribute to decreased MK ploidy.
  • LOX-PP may be a key factor in regulating MK polyploidization, potentially inhibiting it in aberrantly proliferating MKs.
Keywords:
cyclinlysyl oxidase propeptidemegakaryocyteplateletpolyploidy

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