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[Application of a new compact and cell dense continuous culture system for LAK cells generated from cryo-preserved
Y Nakamura1, S Wakamatsu, N Kobayashi
1Blood Transfusion Service Center, Tokai University Hospital.
Nihon Gan Chiryo Gakkai Shi
|March 20, 1990
Abstract:
Recently, the adoptive immunotherapy using lymphokine activated killer (LAK) cells has been applied for various cancer patients. It is quite useful to generate LAK cells from cryo-preserved lymphocytes in order to make a rational treatment schedule. We made basic studies on the induction of LAK cells from frozen lymphocytes using our high yield continuous cell culture system. It was demonstrated that our culture bag can be used to proliferate frozen lymphocytes cultured in interleukin-2 (IL-2) to thirty four-fold the initial cell number in 14 days with comparable cytotoxicity to that of fresh lymphocytes cultured in the same system.