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Orthotopic Small Bowel Transplantation in Rats
Published on: November 6, 2012
Small intestinal submucosa as a graft to increase rectum diameter
Fernando Hintz Greca1, Lucia de Noronha, Fayrus Rodrigo Nastally Marcolini
1Department of Surgery, Pontifical Catholic University of Paraná, Curitiba, Brazil. greca.fernando@gmail.com
The Journal of Surgical Research
|March 26, 2013
Summary
Porcine small intestine submucosa patches significantly increased rectal diameter in rabbits compared to transverse coloplasty. This biocompatible graft demonstrated excellent regeneration and tissue coverage by day 30.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Surgical Innovation
Background:
- Assessing the biocompatibility of porcine small intestine submucosa (PSIS) for colorectal surgery.
- Comparing PSIS graft efficacy to traditional transverse coloplasty in increasing rectal diameter.
Purpose of the Study:
- To evaluate the biocompatibility and efficacy of PSIS as a graft material.
- To compare the ability of PSIS to increase rectal diameter versus transverse coloplasty.
Main Methods:
- 36 New Zealand male rabbits were divided into four groups: two treated with transverse coloplasty (C1, C2) and two with PSIS patches (S1, S2).
- Animals were assessed at 7 and 30 days post-operation for survival, clinical course, anastomosis bursting pressure, morphometrics, histology, and immunohistochemistry.
Main Results:
- PSIS patches significantly increased colonic diameter by day 30 (S2 group).
- No significant difference in anastomosis bursting pressure was observed between PSIS and coloplasty groups.
- Histology at 30 days showed complete epithelial coverage and organized smooth muscle layer formation over the PSIS graft.
Conclusions:
- Porcine small intestine submucosa graft is superior to transverse coloplasty for increasing distal colon diameter.
- The PSIS graft exhibited significant regeneration, fibroplasia, and epithelialization by 30 days post-implantation.
