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Related Experiment Video

Updated: May 13, 2026

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

A programmable microenvironment for cellular studies via microfluidics-generated double emulsions.

Ying Zhang¹, Yi-Ping Ho, Ya-Ling Chiu

¹Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.

|March 26, 2013

Summary

This summary is machine-generated.

Water-in-oil-in-water double emulsions offer a dynamic microenvironment for cell studies. This technology enables continuous nutrient supply and complex biological analysis, overcoming limitations of single emulsions.

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Last Updated: May 13, 2026

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Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli

Published on: August 13, 2016

Area of Science:

Biotechnology
Microfluidics
Cell Biology

Background:

High-throughput cellular studies demand reduced sample volumes for cost-efficiency and enhanced sensitivity.
Microfluidics-based single water-in-oil (W/O) emulsion droplets offer discrete microenvironments but lack continuous nutrient supply and aqueous phase compatibility.
Water-in-oil-in-water (W/O/W) double emulsions address these limitations by encapsulating W/O droplets within an aqueous phase.

Purpose of the Study:

To explore the application of W/O/W double emulsion droplets for advanced cellular studies.
To demonstrate the advantages of double emulsions for dynamic cell cultivation and analysis.
To investigate the potential for studying complex biological events, such as bacterial quorum-sensing, within W/O/W systems.

Main Methods:

Generation of W/O/W double emulsion droplets using microfluidic techniques.
Utilizing the W/O/W system for cell cultivation and genetic activation experiments.
Applying the W/O/W system to study bacterial quorum-sensing dynamics.

Main Results:

W/O/W double emulsions prevent desiccation and minimize organic components while maintaining compartmentalization.
The internal droplet environment can be dynamically programmed without droplet rupture.
Successful demonstration of cell cultivation, genetic activation, and bacterial quorum-sensing studies within W/O/W droplets.

Conclusions:

W/O/W double emulsions provide a versatile platform for cell culture and screening.
This technology enables dynamic control over the cellular microenvironment for complex biological investigations.
Double emulsions represent a significant advancement for studying intricate biological processes in a controlled manner.