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Updated: May 13, 2026

Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation

Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation

Published on: January 12, 2024

Arabidopsis chloroplast quantitative editotype.

Hannes Ruwe¹, Benoit Castandet, Christian Schmitz-Linneweber

¹Molekulare Genetik, Institut für Biologie, Humboldt-Universität zu Berlin, Berlin, Germany.

|March 26, 2013

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Chloroplast C-to-U RNA editing is partial in Arabidopsis thaliana, with some sites missed. A mutant lacking polynucleotide phosphorylase reveals novel editing sites removed by normal RNA processing.

Area of Science:

Plant Molecular Biology
RNA Biology
Genetics

Background:

Chloroplast C-to-U RNA editing is a crucial post-transcriptional modification in plants.
This process ensures the correct expression of chloroplast-encoded genes.

Purpose of the Study:

To investigate the extent and characteristics of RNA editing in Arabidopsis thaliana.
To identify novel RNA editing sites and understand their regulation.

Main Methods:

Strand-specific deep sequencing of RNA from wild-type and mutant Arabidopsis thaliana.
Analysis of RNA editing patterns in a mutant defective in RNA 3' end maturation.

Main Results:

All analyzed RNA editing sites exhibit partial editing, with 5-6% of RNAs unedited.

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Last Updated: May 13, 2026

Non-invasive Assay for Chlorophyll Biosynthesis Kinetics Determination during Early Stages of Arabidopsis De-etiolation

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Published on: January 12, 2024

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Investigating Tissue- and Organ-specific Phytochrome Responses using FACS-assisted Cell-type Specific Expression Profiling in Arabidopsis thaliana

Published on: May 29, 2010

Nine novel RNA editing sites were identified, showing low editing extents.

Three novel sites, normally removed by 3' end processing, accumulate and are edited in a polynucleotide phosphorylase-deficient mutant.

Conclusions:

Chloroplast RNA editing is a dynamic and incompletely understood process.
RNA 3' end processing plays a role in regulating the accessibility and editing of certain RNA regions.