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Derivatization in liquid chromatography for mass spectrometric detection
1Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
Drug Discoveries & Therapeutics
|March 26, 2013
Summary
This review covers derivatization reagents used in liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) for analyzing low molecular weight compounds. It focuses on methods enhancing detection sensitivity in biological samples from 2008-2012.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Mass Spectrometry
Background:
- Liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) is vital for trace compound analysis in biological matrices.
- Enhancing analyte detection sensitivity is crucial for accurate quantification.
- Chemical derivatization is a key strategy to improve LC/ESI-MS/MS performance.
Purpose of the Study:
- To provide a comprehensive overview of derivatization reagents used in LC/ESI-MS/MS.
- To focus on applications for low molecular weight compounds.
- To cover literature published between 2008 and 2012.
Main Methods:
- Literature review of scientific publications.
- Analysis of derivatization reagents and their impact on LC/ESI-MS/MS.
- Focus on methods applied to low molecular weight analytes.
Main Results:
- Identified various derivatization reagents that improve sensitivity and selectivity in LC/ESI-MS/MS.
- Highlighted the application of these reagents for trace analysis of small molecules.
- Summarized trends in derivatization strategies over the 2008-2012 period.
Conclusions:
- Derivatization is an effective technique for enhancing LC/ESI-MS/MS analysis of low molecular weight compounds.
- The selection of appropriate derivatization reagents is critical for sensitive and selective detection.
- Continued development of derivatization strategies will advance trace analysis in biological samples.
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